Evaluation of Better Staining Method among Hematoxylin and Eosin, Giemsa and Periodic Acid Schiff-Alcian Blue for the Detection of Helicobacter pylori in Gastric Biopsies

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A new staining method using a hematoxylin, alcian blue and periodic acid-Schiff reaction for demonstrating mucins in gastric glands.

Mucous neck cells in the rat stomach which contain neutral mucin were stained with a modified Mayer's hemalum. When alcian blue staining and periodic acid-Schiff (PAS) reaction preceded hematoxylin staining, mucin-containing fundic gland cells could be distinguished in the same tissue sections: surface mucous cells were positive for PAS reaction and/or alcian blue staining, neutral mucin-contai...

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The Immunohistochemistry and Toluidine Blue Roles for Helicobacter pylori Detection in Patients with Gastritis

Background: Helicobacter pylori, which is associated with many upper gastrointestinal diseases, is found in half of the population of the world. Several special stains and immunohistochemistry stain for H. pylori are available. The need for and usefulness of immunohistochemical (IHC) technique has been debated for years. Toluidine blue is a simple stain for microbiological studies and is easily...

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مقایسه سه نوع رنگ‌آمیزی هماتوکسیلین و ائوزین، گیمسا و هماتوکسیلین و ائوزین با هماتوکسیلین طولانی مدت برای تشخیص هلیکوباکترپیلوری در بیوپسی‌های معده افراد مبتلا به گاستریت مزمن

    Background & Aim: Regarding the high incidence of upper GI(gastrointestinal) inflammatory diseases and the clear role of helicobacter pylori in their pathogenesis, this study was designed to evaluate the ability of different staining methods in detection of this bacterium. Material & Method: In an analytical cross-sectional study, 50 patients suffering from upper GI symptoms who were referr...

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ژورنال

عنوان ژورنال: Malaysian Journal of Medical Sciences

سال: 2020

ISSN: 1394-195X,2180-4303

DOI: 10.21315/mjms2020.27.5.6